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bovine pituitary extract (bpe) for ksfm medium  (Thermo Fisher)


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    Thermo Fisher bovine pituitary extract (bpe) for ksfm medium
    Bovine Pituitary Extract (Bpe) For Ksfm Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ksfm/bovine+pituitary+extract/pmc12212142-27-5-8
    Average 90 stars, based on 1 article reviews
    bovine pituitary extract (bpe) for ksfm medium - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Exosome-delivered NR2F1-AS1 and NR2F1 drive phenotypic transition from dormancy to proliferation in treatment-resistant prostate cancer via stabilizing hormonal receptors.
    Article Snippet: PCa cells were grown in 10% fetal bovine serum (FBS; Hyclone, USA) supplemented RPMI 1640 media (Gibco, USA), while RWPE1 cells were maintained in KSFM (Gibco, no. 10744-019).

    Cell Culture:

    Article Title: Multiple cell types support productive infection and dynamic translocation of infectious Ebola virus to the surface of human skin
    Article Snippet: .. Cells were washed with 2% FBS in DPBS and centrifuged at 200 g for 10 min and cultured in KSFM (Thermo Fisher Scientific, #17005042), which inhibits the growth of non-keratinocyte cells, resulting in a pure population of keratinocytes after passaging. ..

    Article Title: Adipose-derived mesenchymal stromal cells: A study on safety and efficacy in ocular inflammation.
    Article Snippet: Purpose: This study explores the application of adipose-derived mesenchymal stromal cells (adMSCs) as a therapy for ocular inflammatory diseases utilizing a chronic GVHD model. Methods: Human adMSCs were administered via subconjunctival injection into mice with chronic ocular GVHD.. Clinical scores and changes in T cell populations were analyzed.. Results: The study showed significant improvement in corneal integrity, including epithelial damage, opacity, thickness, and structure, after subconjunctival adMSC transplantation.

    Article Title: Olfactory receptors impact pathophysiological processes of lung diseases in bronchial epithelial cells.
    Article Snippet: .. The cells were cultured in KSFM (keratinocyte-SF-medium, Gibco, cat: 17005042) supplemented with human epidermal growth factor (Gibco) at a concentration of 2.5 ng/ml, bovine pituitary extract (Gibco) at a concentration of 25 μg/ml, isoproterenol (Sigma-Aldrich, cat: I6504) at a concentration of 1 μM, and penicillin/streptomycin, and amphotericin B at a concentration of 2.5 μg/ml. ..

    Article Title: ERK5 suppression overcomes FAK inhibitor resistance in mutant KRAS-driven non-small cell lung cancer
    Article Snippet: A549 (CCL-185), HEK293T (CRL-11268) and Phoenix-Ampho cells (CRL-3213) cell lines were purchased from ATCC. .. The HBEC3-KT cells were cultured in KSFM (Gibco) supplemented with EGF, Bovine Pituitary extract (Gibco) (Ramirez et al, ). .. The human NSCLC cell lines A549, A427 and H460 were cultured in RPMI-1640 (Gibco) supplied with 10% FBS (Thermo Fisher), 100 I.U./mL penicillin and 100 μg/mL streptomycin (Gibco).

    Passaging:

    Article Title: Multiple cell types support productive infection and dynamic translocation of infectious Ebola virus to the surface of human skin
    Article Snippet: .. Cells were washed with 2% FBS in DPBS and centrifuged at 200 g for 10 min and cultured in KSFM (Thermo Fisher Scientific, #17005042), which inhibits the growth of non-keratinocyte cells, resulting in a pure population of keratinocytes after passaging. ..

    Recombinant:

    Article Title: A cellular disease model toward gene therapy of TGM1 -dependent lamellar ichthyosis
    Article Snippet: Low-calcium medium contained KSFM (17005042, Thermo Fisher Scientific) supplemented with 0.03 mM calcium chloride, 25 μg/mL bovine pituitary extract (BPE; Thermo Fisher Scientific), 0.2 ng/mL of recombinant EGF (Thermo Fisher Scientific), and penicillin-streptomycin (100 IU/mL). .. High-calcium medium contained KSFM supplemented with 2.8 mM of calcium chloride, 25 μg/mL BPE, 0.2 ng/mL of recombinant EGF (Thermo Fisher Scientific), and penicillin-streptomycin (100 IU/mL). .. Genomic DNA from human primary keratinocytes (K81) was used as template to generate (1i) TGM1 min , (2) IVL min , and (3) TGM1 full by PCR using high-fidelity Taq polymerase (LA Taq DNA Polymerase, Takara).

    Concentration Assay:

    Article Title: Olfactory receptors impact pathophysiological processes of lung diseases in bronchial epithelial cells.
    Article Snippet: .. The cells were cultured in KSFM (keratinocyte-SF-medium, Gibco, cat: 17005042) supplemented with human epidermal growth factor (Gibco) at a concentration of 2.5 ng/ml, bovine pituitary extract (Gibco) at a concentration of 25 μg/ml, isoproterenol (Sigma-Aldrich, cat: I6504) at a concentration of 1 μM, and penicillin/streptomycin, and amphotericin B at a concentration of 2.5 μg/ml. ..



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    Image Search Results


    PAX6, TP53, TP63, FOSL2, FOXC1, PPARγ and NFκB mRNA expression (A) and PAX6 and FOSL2 protein levels (B) in LECs transfected with FABP5 siRNA or control (ctrl) siRNA. Western blot image from a single biological replicate (C) . Data are shown as mean ± SEM. Statistical analysis has been performed using two-way ANOVA followed by Tukey test (n = 7). p-values below 0.05 were considered statistically significant. PAX6 mRNA level was significantly lower in FABP5 knockdown LECs than in ctrl siRNA LECs, in KSFM medium or following LPS and IL-1β treatment (p ≤ 0.022). Furthermore, PAX6 protein level was significantly lower following FABP5 knockdown, than in ctrl siRNA LECs without treatment and after IL-1β treatment (p ≤ 0.031). TP63 mRNA level was significantly higher in ctrl siRNA LECs following LPS or IL-1β treatment, than without treatment (p ≤ 0.015). FOSL2 mRNA level was significantly higher in ctrl siRNA LECs following IL-1β treatment, than without treatment (p = 0.033). Under LPS and IL-1β treatment, FOSL2 mRNA levels were significantly lower in FABP5 knockdown cells than in control (transfected) cells (p ≤ 0.025).

    Journal: PLOS One

    Article Title: Fatty acid-binding protein 5 (FABP5) modulates limbal epithelial cell homeostasis by regulating the expression of key genes under both normal and inflammatory conditions , in vitro

    doi: 10.1371/journal.pone.0347228

    Figure Lengend Snippet: PAX6, TP53, TP63, FOSL2, FOXC1, PPARγ and NFκB mRNA expression (A) and PAX6 and FOSL2 protein levels (B) in LECs transfected with FABP5 siRNA or control (ctrl) siRNA. Western blot image from a single biological replicate (C) . Data are shown as mean ± SEM. Statistical analysis has been performed using two-way ANOVA followed by Tukey test (n = 7). p-values below 0.05 were considered statistically significant. PAX6 mRNA level was significantly lower in FABP5 knockdown LECs than in ctrl siRNA LECs, in KSFM medium or following LPS and IL-1β treatment (p ≤ 0.022). Furthermore, PAX6 protein level was significantly lower following FABP5 knockdown, than in ctrl siRNA LECs without treatment and after IL-1β treatment (p ≤ 0.031). TP63 mRNA level was significantly higher in ctrl siRNA LECs following LPS or IL-1β treatment, than without treatment (p ≤ 0.015). FOSL2 mRNA level was significantly higher in ctrl siRNA LECs following IL-1β treatment, than without treatment (p = 0.033). Under LPS and IL-1β treatment, FOSL2 mRNA levels were significantly lower in FABP5 knockdown cells than in control (transfected) cells (p ≤ 0.025).

    Article Snippet: After centrifugation at 200g for 5 minutes, cells were cultured in Keratinocyte growth medium (KSFM) (Cat. Nr. C-20111, Promocell, Heidelberg, Germany), at 37 oC, 5% CO 2 .

    Techniques: Expressing, Transfection, Control, Western Blot, Knockdown